Bio::Tools::Genemark(3p)

NAME
Bio::Tools::Genemark - Results of one Genemark run

SYNOPSIS
   $Genemark = Bio::Tools::Genemark->new(-file => 'result.Genemark');
   # filehandle:
   $Genemark = Bio::Tools::Genemark->new( -fh  => \*INPUT );

   # parse the results
   # note: this class is-a Bio::Tools::AnalysisResult which implements
   # Bio::SeqAnalysisParserI, i.e., $Genemark->next_feature() is the same
   while($gene = $Genemark->next_prediction()) {
       # $gene is an instance of Bio::Tools::Prediction::Gene, which inherits
       # off Bio::SeqFeature::Gene::Transcript.
       #
       # $gene->exons() returns an array of
       # Bio::Tools::Prediction::Exon objects
       # all exons:
       @exon_arr = $gene->exons();

       # initial exons only
       @init_exons = $gene->exons('Initial');
       # internal exons only
       @intrl_exons = $gene->exons('Internal');
       # terminal exons only
       @term_exons = $gene->exons('Terminal');
       # singleton exons:
       ($single_exon) = $gene->exons();
   }

   # essential if you gave a filename at initialization (otherwise the file
   # will stay open)
   $Genemark->close();

DESCRIPTION
The Genemark module provides a parser for Genemark gene structure prediction output. It parses one gene prediction into a Bio::SeqFeature::Gene::Transcript- derived object.

This module has been developed around genemark.hmm for eukaryots v2.2a and will probably not work with other versions.

This module also implements the Bio::SeqAnalysisParserI interface, and thus can be used wherever such an object fits. See Bio::SeqAnalysisParserI.

FEEDBACK

  Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.

  bioperl-l@bioperl.org                  - General discussion
  http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

  Support
Please direct usage questions or support issues to the mailing list:

bioperl-l@bioperl.org

rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.

  Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:

  https://github.com/bioperl/bioperl-live/issues

AUTHOR - Hilmar Lapp, Mark Fiers
Email hlapp@gmx.net
      m.w.e.j.fiers@plant.wag-ur.nl

APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

  new
 Title   : new
 Usage   : my $obj = Bio::Tools::Genemark->new();
 Function: Builds a new Bio::Tools::Genemark object
 Returns : an instance of Bio::Tools::Genemark
 Args    : seqname

  analysis_method
 Usage     : $Genemark->analysis_method();
 Purpose   : Inherited method. Overridden to ensure that the name matches
             /GeneMark.hmm/i.
 Returns   : String
 Argument  : n/a

  next_feature
 Title   : next_feature
 Usage   : while($gene = $Genemark->next_feature()) {
                  # do something
           }
 Function: Returns the next gene structure prediction of the Genemark result
           file. Call this method repeatedly until FALSE is returned.

           The returned object is actually a SeqFeatureI implementing object.
           This method is required for classes implementing the
           SeqAnalysisParserI interface, and is merely an alias for
           next_prediction() at present.

 Example :
 Returns : A Bio::Tools::Prediction::Gene object.
 Args    :

  next_prediction
 Title   : next_prediction
 Usage   : while($gene = $Genemark->next_prediction()) {
                  # do something
           }
 Function: Returns the next gene structure prediction of the Genemark result
           file. Call this method repeatedly until FALSE is returned.

 Example :
 Returns : A Bio::Tools::Prediction::Gene object.
 Args    :

  _parse_predictions
 Title   : _parse_predictions()
 Usage   : $obj->_parse_predictions()
 Function: Parses the prediction section. Automatically called by
           next_prediction() if not yet done.
 Example :
 Returns :

  _prediction
 Title   : _prediction()
 Usage   : $gene = $obj->_prediction()
 Function: internal
 Example :
 Returns :

  _add_prediction
 Title   : _add_prediction()
 Usage   : $obj->_add_prediction($gene)
 Function: internal
 Example :
 Returns :

  _predictions_parsed
 Title   : _predictions_parsed
 Usage   : $obj->_predictions_parsed
 Function: internal
 Example :
 Returns : TRUE or FALSE

  _has_cds
 Title   : _has_cds()
 Usage   : $obj->_has_cds()
 Function: Whether or not the result contains the predicted CDSs, too.
 Example :
 Returns : TRUE or FALSE

  _read_fasta_seq
 Title   : _read_fasta_seq()
 Usage   : ($id,$seqstr) = $obj->_read_fasta_seq();
 Function: Simple but specialised FASTA format sequence reader. Uses
           $self->_readline() to retrieve input, and is able to strip off
           the traling description lines.
 Example :
 Returns : An array of two elements.

  _seqname
 Title   : _seqname
 Usage   : $obj->_seqname($seqname)
 Function: internal
 Example :
 Returns : String